Experimental study on microencapsulated NGF expressing NIH3T3 cells on repair of peripheral nerve defect in rats
- VernacularTitle:微囊化转NGF基因NIH3T3细胞填充静脉修复大鼠坐骨神经缺损的作用
- Author:
Jingguo ZHAO
;
Ju ZHANG
;
Fengtong GAO
- Publication Type:Journal Article
- Keywords:
peripheral nerves;
gene altered cell;
microencapsules;
nerve regeneration;
nerve growth factor
- From:
Journal of Jilin University(Medicine Edition)
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of microencapsulated NGF expressing NIH3T3 cells on the repair of peripheral nerve defect in rats.Methods Thirty SD male rats with 10 mm defect of sciatic nerve were randomly divided into three groups(n=10).Group A: The nerve defect was bridged with heterogeneous nerve,the neural regeneration room was formed and filled with microencapsulated NGF expressing NIH3T3 cells.Group B: Autogenous nerve grafting was performed.Group C: The nerve defect was bridged with heterogeneous nerve,the neural regeneration room was formed and filled with microencapsulated NIH3T3 cells.The walking track analysis,electrophysiological and morphological observation were performed 12 weeks after operation.Results There were no significant differences of sciatic nerve function index,nerve concluctive velocity,histological changes of regenerative nerve,maturation degree of regenerative nerve fiber between group A and group B 4,8,12 weeks after operation;group A and B were surperior to group C(P
