Development and clinical application of vWF-pp ELISA Kit
- VernacularTitle:血管性血友病因子前肽双抗体夹心法检测及临床应用
- Author:
Jiju HAN
;
Daoling REN
;
Bin CHEN
;
Zengxian WANG
;
Ran WEI
;
Yaping WU
- Publication Type:Journal Article
- Keywords:
von Willebrand disease;
Enzyme-linked immunosorbent assay
- From:
Chinese Journal of Laboratory Medicine
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a sandwich enzyme linked immunosorbent assay (ELISA) method for determination of von Willebrand factor propeptide (vWF-pp) in plasma and to determine the levels of plasma vWF-pp in 152 healthy volunteers, 36 patients with coronary heart disease and 38 cases of the normal control group with the same ages.Methods The levels of vWF-pp in 152 healthy volunteers with different ages and different genders and in 36 patients with coronary heart disease were determined with the sandwich enzyme linked immuno-sorbent assay (ELISA).Results The average level of vWF-pp in 152 healthy volunteers was 496.4?167.2 ?g/L.The level of vWF-pp in the 50~60 years old group was significantly higher than that in the less than 40 years old group (P0.05). There was significant difference between the 51～60 years old male group and the other three male groups respectively, the 16～20 years old group, the 21～30 years old group and the 31～40 years old group (P
