Construction and characterization of hfgl 2 expression vector
- VernacularTitle:人纤维介素基因真核表达载体的构建及表达
- Author:
Li LI
;
Chuanlong ZHU
;
Qin NING
- Publication Type:Journal Article
- Keywords:
Fibrinogen-like protein 2 gene;
Gene expression;
Viral hepatitis
- From:
Journal of Medical Postgraduates
2003;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct hfgl 2 expression vector(pcDNA3.1-hfgl 2) and characterize the expression of hfgl 2 in CHO cells after transfection. Methods: Total RNA was extracted from chinese human peripheral blood monocyte cells,cDNA was obtained by reverse transcription,hfgl 2 cDNA was amplified and cloned into pcDNA3.1 and the orientation and the sequence were ensured by restriction endonucleases and sequencing assays.The recombinated plasmid was transfected into CHO cells,and the expression of hfgl 2 was detected by immunohistochemistry. Results:A 1.3 kb long target fragment was obtained and cloned into pcDNA3.1.The orientation and sequence are correct.hfgl 2 was only expressed in those cells transfected with pcDNA3.1-hfgl 2. Conclusion:hfgl 2 expression vector(pcDNA3.1-hfgl 2) has been successfully constructed.
