The Study for Chromosome 3p Loss in Renal Cell Carcinoma by Fluorescence in Situ Hybridization Using Paraffin-Embedded Tissue.
- Author:
Hyeon JEONG
1
;
Jin Ren JIE
;
Jin Soo CHUNG
;
Cheol KWAK
;
Dae Young KIM
;
Seung Bae LEE
;
Sang Eun LEE
Author Information
1. Department of Urology, Seoul National University College of Medicine, Korea.
- Publication Type:Original Article
- Keywords:
Renal cell carcinoma;
Deletion of 3p;
Fluorescence in situ hybridization
- MeSH:
Arm;
Carcinogenesis;
Carcinoma, Renal Cell*;
Chromosomes, Human, Pair 2;
Classification;
Cytogenetics;
DNA;
Fluorescence*;
Humans;
In Situ Hybridization*;
In Situ Hybridization, Fluorescence;
Kidney;
Molecular Biology;
Paraffin;
Ploidies
- From:Korean Journal of Urology
1999;40(6):697-702
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Conventional pathologic classifications of human renal cell carcinoma give little insight into oncogenesis and little assistance in predicting the clinical behavior of this disease. For genetic classification, deletion of the short arm of chromosome 3(3p), the hallmark of nonpapillary/clear cell RCC, is a major diagnostic criterion. Because of the limited routine applicability of cytogenetics and molecular genetic techniques we investigated fluorescence in situ hybridization(FISH) for the detection of this aberration in RCC. MATERIALS AND METHODS: Isolated nuclei from 8 human RCC paraffin embedded tissue sections were examined with a dual color FISH technique for loss of chromosome 3p. Telomeric DNA probe from 3p and an internal ploidy control probe, centromeric probe of chromosome 2, were applied to the isolated nuclei of RCC. RESULTS: 87.5% of the patients(7) lost chromosome 3p. The loss of 3p in the samples tested was unrelated to patient age, gender, tumor stage, and grade. CONCLUSIONS: FISH for the detection of loss in 3p from paraffin embedded tissue sections provides a sensitive and feasible methods for the genetic classification of kidney tumors and FISH is a very useful diagnostic tool for detection of the genetic aberrations of the tumors.
