Quantitative detection of Rabies virus antibody by chemiluminescent immunoassay
- VernacularTitle:化学发光免疫定量检测狂犬病毒抗体方法的建立
- Author:
Lin SONG
;
Lanying GUO
- Publication Type:Journal Article
- Keywords:
Rabies virus;
chemiluminescent immunoassay;
enzyme linked immunoadsorbent assay
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish chemiluminescent immunoassay(CLIA) for quantitative detection of Rabies virus antibody in human serum.Method The purified antigen was coated on the microplate,pairing with alkaline phosphatase(ALP)-labeled antigen and luminescence substrate CSPD,then the double antigen sandwich CLIA was established.Results The sensitivity of the assay was 0.2IU/ml.The linear range was from 0 IU/ml to 200 IU/ml.The precision was less than 10%.The analytical recovery rate was from 90% to 110%.Conclusion The CLIA is a simple,sensitive,special and repeatable method for detection of Rabies virus antibody.It was suitable for clinical application.
