Detection for expression level of gene FN1,NME2 and TIMP3 in mesenchymal stem cells,tumor cells and leukemia cells
- VernacularTitle:骨髓间质干细胞与肿瘤细胞中FN1、NME2、TIMP3基因表达检测
- Author:
Weike CAO
- Publication Type:Journal Article
- Keywords:
gene;
mesenchymal stem cells;
F6;
leukemia
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the differential expression of genes encoding fibronectin 1(FN1),non-metastatic cell proteins 2(NME2) and tissue inhibitor of metalloproteinase 3(TIMP3) in fetal bone marrow mesenchymal stem cells(FMSCs),adult bone marrow mesenchymal stem cells(AMSCs),F6,SGC and leukemia cells and study the molecular biological characters of tumor and leukemia cells.Methods RT-PCR was used to clone the cDNA of total RNA extracted from FMSCs,AMSCs,F6,SGC and leukemia cells respectively and then the expression levels of gene FN1,NME2 and TIMP3 were detected by PCR and real-time PCR.Results The expression levels of gene FN1,NME2 and TIMP3 in AMSCs were higher than those in FMSCs,F6,SGC(P
