Effects of iow-density lipoprotein-containing immune complexes on matrix metalloproteinase-1 and tissue inhibitors of metalloproteinase-1 in U937 cells
- VernacularTitle:低密度脂蛋白循环免疫复合物对U937细胞基质金属蛋白酶-1及其抑制物的作用
- Author:
Zhenhua GU
;
Junjun WANG
;
Yufeng HUANG
;
Lian LIU
;
Chunni ZHANG
- Publication Type:Journal Article
- Keywords:
iow-density lipoprotein-containing;
immune complexes;
U937 cells;
MMP-1;
TIMP-1;
gene expression
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of low density lipoprotein(LDL) containing immune complexes(IC) on the mRNA expression of matrix metalloproteinase-1(MMP-1) and matrix metalloproteinase-1(TIMP-1) in U937 cells.Methods U937 cells were incubated with native LDL,oxidized LDL or LDL-IC,respectively.mRNA expression of matrix metalloproteinase-1(MMP-1) and tissue inhibitor of metalloproteinase-1(TIMP-1) in U937 cells were measured by reverse transcriptase polymerase chain reaction(PT-PCR).Results Normal U937 cells hardly expressed MMP-1,but highly expressed TIMP-1.N-LDL had no effect on the expression of MMP-1 and TIMP-1 mRNA.Ox-LDL can induce slightly expression of MMP-1 and markedly down-regulate expression of TIMP-1.Similarly,nature and oxidized LDL-IC had significantly more effect on the mRNA expressions of MMP-1 and TIMP-1 than that of control(P
