Inhibitory effect of RRTJ and NoniJ on growth of human ovarian cancer cells
- VernacularTitle:刺梨汁和诺丽汁对人卵巢癌细胞株COC_2抑制作用的研究
- Author:
Guoping XU
- Publication Type:Journal Article
- Keywords:
RRTJ;
NoniJ;
COC_2;
growth inhibition
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effects of rose roxburghii tratt juice(RRTJ) and morinda citrifolia juice(NoniJ) on the growth of human ovarian cancer cell line COC_2 in vitro.Methods Three treatment groups(RRTJ alone,NoniJ alone,RRTJ plus NoniJ) were used respectively to incubate with COC_2 cells and peripheral blood mononuclear cells.Inhibitory effect of juice on cells was determined by counting the living cells.Flow CytoMeter(FCM) was used to investigate the cell cycle,and Hoechst 33258 fluorescent staining method for examining the cellular apoptosis.LDH released into medium was measured to evaluate the virulent effect of juice on the mononuclear cells.Results The inhibition of RRTJ to the growth of COC_2 was dose-dependent.After incubating with RRTJ(concentration from 4 ?l/ml-16 ?l/ml) for 48 h,the cells were arrested in G0/G1 phase(P
