Effects of D-Amino Acid Oxidase on Chiral Inversion of N~G-nitro-D-arginine
- VernacularTitle:D型氨基酸氧化酶活性对于D-硝基精氨酸手性转化的影响
- Author:
Yanfei XIN
;
Xiangjun ZHOU
;
Yongxiang WANG
- Publication Type:Journal Article
- Keywords:
NG-nitro-D-arginine (D-NNA), D-amino acid oxidase (DAAO), D-amino acids, chiral inversion
- From:
Progress in Biochemistry and Biophysics
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
NG-nitro-D-arginine (D-NNA) produced pressor responses in rats by acting via chiral inversion intoNG-nitro-L-arginine (L-NNA), an inhibitor of nitro oxide synthase. The present investigation aimed to study the roleof the D-amino acid oxidase (DAAO) in chiral inversion of D-NNA and the relationship between DAAO activitieson various D-amino acids and their inversion rate. Benzoate (400 mg/kg) or creatinine (400 mg/kg), two inhibitorsof DAAO, blocked D-NNA-induced pressor responses in rats. Furthermore, the addition of the pure DAAOsignificantly potentiates L-NNA production rate in kidney homogenates by approximately 2-folds. The in vivo andin vitro results suggested that DAAO plays an essential role in the pressor responses elicited by D-NNA.Moreover, crude DAAO solution from the kidney showed marked selection (the maximal ratio of Kcat/Km wasnearly 15 times) on different D-amino acids that exhibited similar chiral inversion rate in vivo, suggesting that otherenzymes, such as transaminase, are also required for the entire process of D-NNA chiral inversion.
