Studies on The Interactions Between NIRF and P53
- VernacularTitle:NIRF对P53蛋白泛素化作用的研究
- Author:
Changzhu DUAN
;
Shuping PU
;
Mori TSUTOMU
;
Kochi HIDEO
;
Zongyin QIU
- Publication Type:Journal Article
- Keywords:
NIRF, P53, GST pull-down, ubiquitination
- From:
Progress in Biochemistry and Biophysics
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
HEK293 or HeLa cells were transfected by NIRF and, or P53, whole cell extracts andimmunoprecipitates were subjected to SDS-PAGE followed by Western blotting. GST pull-down was carried outto identify the interactions between NIRF and P53. In vitro ubiquitination reaction was carried out to identify P53ubquitinate by NIRF. The results suggested that NIRF could interact with P53 in vivo and in vitro. The results alsoshowed that NIRF could ubiquitinate P53 in vivo and in vitro. The results indicated that NIRF would be a newnegative regulator of P53.
