CCl_4 promotes the proliferation and expression of TGF?1 in rat hepatic stellate cells
- VernacularTitle:CCl_4促进大鼠肝星状细胞增殖及TGF-?1基因表达
- Author:
Qinghua GAO
- Publication Type:Journal Article
- Keywords:
CCl_(4);
TGF-?1;
hepatic stellate cell
- From:
Basic & Clinical Medicine
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular mechanism of CCl_(4) inducing fibrosis in hepatic stellate cells.Methods The expression of Transforming growth factor-?1(TGF-?1) at mRNA and protein level were detected by RT-PCR method and Western blot analysis.The proliferation and cell cycle distribution were detected with MTT method and flow cytometry.Results After treatment by(10 mmol/L) CCL_(4) for 24 hours,the mRNA and protein expression of TGF-?1 in CCl_(4) group sharply increased.In the meantime,the proliferation of CCl_(4) group cells was markedly increased.There were less G_(0)/G_(1) phase_() cells and more G_(2)/M phase cells in CCl_(4) group than those in control group.Conclusion Suitable CCl_(4) may increase the expression of TGF-?1 and the proliferation of hepatic stellate cells,which is one of mechanisms leading to hepatic fibrosis.
