Survivin gene-targeted siRNA inducing apoptosis of human osteosarcoma cell line U-2OS
- VernacularTitle:靶向生存素基因siRNA诱导骨肉瘤细胞株U-2OS的凋亡
- Author:
Jia WANG
;
Gang LU
;
Hailun GU
;
Yanfen WANG
- Publication Type:Journal Article
- Keywords:
osteosarcoma;
RNA interference;
siRNA;
survivin gene;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the proliferation and apoptosis of human osteosarcoma cell line U-2OS after sur-vivin gene was knocked down by siRNA synthesized in vitro. Methods: U-2OS cells were divided into 3 groups: Group A (blank control) , group B ( transfected with non-specific siRNA) and group C( transfected with survivin-specific siRNA). The morphological changes of U-2OS cells were observed with fluorescent microscope. The expression of survivin mRNA and protein were detected by reverse transcription polymerase chain reaction ( RT-PCR) and Western blotting. The anti-proliferative effects were assessed by MTT and the rate of apoptosis was examined by flow cytometry in 3 groups. The results of the 3 groups were analyzed and compared. Results: Survivin specific siRNA significantly down-regulated mRNA and protein expression of survivin. Expression of survivin protein in group C was significantly lower than those in group A and B( P
