Application of FISH technique for detection of fusion gene ABL/BCR in chronic myelogenous leukemia
- Author:
Vinh Quang Pham
;
Hoang Cong Tran
;
Cuong Quoc Nguyen
;
Hoa Khanh Bach
- Publication Type:Journal Article
- Keywords:
chronic myelogenous leukemia;
abl/bcr fusion gene;
FISH
- MeSH:
Leukemia;
Myelogenous;
Chronic;
BCR-ABL Positive/ pathology
- From:Journal of Medical Research
2007;51(4):35-40
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Background: Detection of BCR/ABL fusion gene has important significance in diagnosing and monitoring response to therapy in chronic myeloid leukemia. Objective: Application of FISH (Fluorescence In Situ Hybrydization) technique for detection of abl/bcr fusion gene in chronic myelogenous leukemia. Subjects and methods: The study included 10 patients of chronic myelogenous leukemia diagnosed by methods of morphology and cell chemistry. Peripheral blood and bone marrow samples of them were analyzed Philadelphia (Ph1) chromosome by cytogenetic technique. Among them, 5 patients were tested by FISH technique on the slide of interphase and remainders were tested by FISH technique on the slide of metaphase cell. Results: Results of analyzing chromosome of 10 patients showed that 8 patients had Ph1 chromosome. 2 patients without Ph1 chromosome were patients who had not high of leukocyte count: 28x109leukocyte/l and 36x109leukocyte/l, respectively. In the FISH on the slide of interphase, all 5 patients had Ph1 chromosome and abl/bcr fusion gene. In the FISH on the slide of metaphase cell, 3 patients had Ph1 chromosome and abl/bcr fusion gene. Conclusion: FISH technique has been applied successfully to detect ABL/BCR gene in patients with chronic myelogenous leukemia.\r\n', u'\r\n', u'
