Determination of sodium valprate in plasma by capillary zone electrophoresis
- VernacularTitle:毛细管区带电泳测定血浆中丙戊酸钠浓度
- Author:
Yang LIU
;
Shining ZHANG
;
Yingdong ZHANG
- Publication Type:Journal Article
- Keywords:
Sodium valprate;
Plasma concentration;
Electrophoresis,capillary
- From:
Chinese Journal of Laboratory Medicine
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid determination of sodium valprate in plasma by capillary zone electrophoresis.Method Plasma was acidized by 1 mol/L HCl, VPA was extracted into organic phase (n-Hexane), then re-extracted into aqueous phase (0.05 mmol/L NaOH solution including 15% Ethanol). Capillary clone was 75 ?m(id)?37 cm. Electrolyte consisted of 15 mmol/L sodium salicylate, 0.5 mmol/L CTAB and 15% Ethanol (pH 5.7).Separating voltage was 20 kV, detection wave was 214 nm, temperature was 20℃,injection time was 5 s by press in negative.Result The linear ranger of concentration for standard drug was between 25~200 ?g/ml (r=0.999),the limit of detection was 0.35 ?g/ml, the average recovery of VPA was 87.4%,the average inter-day and intar-day CV were less than 4% and 6%. Conclusion This method is simple, rapid, reliable and correct, for determination of VPA in plasma.
