Interference effects of ?-aeacine sodium on the apoptosis and the expression of Akt in neurons after intracerebral hemorrhage in rats
- VernacularTitle:大鼠脑出血后神经细胞凋亡和蛋白激酶B表达及?-七叶皂甙钠的干预作用
- Author:
Yong ZHANG
;
Xinsheng DING
;
Fei GAO
- Publication Type:Journal Article
- Keywords:
intracerebral hemorrhage;
apoptosis;
AKt;
?-aeacine sodium
- From:
Journal of Clinical Neurology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the apoptosis of neuron and protein kinase B (Akt) expression after intracerebral hemorrhage (ICH)in rats, and investigate the interference effect and mechanism of ?-aeacine sodium on ICH injury.Methods All Sprague - Dawley male rats were randomly divided into normal group, pseudo -operation group,model group and therapeutic group treated with ?-sodium aeacine.The latter three groups were divided into five subgroups respectively :6 h,12h,1d,3d and 7d.ICH models were performed by injection Ⅶ type collagenase into the the right globus pallidus.By the methods of in situ terminaldeoxynucleotidyl transferase- mediated dUTP nick end labeling (TUNEL) and immunohistochemistry, The dynamic changes of apoptosis and the expression of AKt protein were observed in the damaged cortex.Results TUNEL-positive cells appeared at 6h after collagenase injection in the model group and the expression of AKt increased at 12 h.They reached their peak at 3rd day, and were still present during 1 week. The numbers of TUNEL-positive cells in 1 d, 3 d, 1 week after ICH decreased markerly in treatment group than those of in model group ( P
