MDR1-siRNA reverses multidrug resistance in KBv200 cells
- VernacularTitle:小分子干扰RNA片段逆转KBv200细胞多药耐药的实验研究
- Author:
Gang FANG
- Publication Type:Journal Article
- Keywords:
siRNA;
Multidrug resistance;
Vincristine;
Doxorubicin
- From:
Journal of Medical Postgraduates
2004;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of siRNA on multidrug resistance1(MDR1) gene expression in KBv200 cells. Methods: MDR1-siRNA was synthesized and transfected into KBv200 cells.The MDR1 mRNA and P-gp were assayed by RT-PCR and flow cytometry.Cytotoxicity was determined by a tetrazolium-based chemosensitivity assay(MTT).Cellular accumulation of Dox was measured by fluorescence spectrophotometry. Results: MDR1-siRNA decreased MDR1 mRNA and P-gp expression(P
