Role of RhoA in the endothelial cell monolayer permeability induced by thrombin or lipopolysaccharide
- VernacularTitle:RhoA介导凝血酶或脂多糖诱导内皮细胞株单层通透性增高
- Author:
Yaling HAN
- Publication Type:Journal Article
- Keywords:
LPS;
thrombin;
HUVEC;
cytoskeleton;
permeability
- From:
Basic & Clinical Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the change of permeability of HUVECs monolayer induced by thrombin or Lipopolysaccharide(LPS) for exploring the role of RhoA.Methods The effect of thrombin or LPS on the permeability of HUVECs monolayer was examined with the polycarbonate transwell filters and the effect of thrombin or LPS on HUVECs F-actin was observed with immunocytochemistry.The interferring action of Y-27632(Rho kinase inhibitor) on the permeability and F-actin change induced by thrombin or LPS was also observed.The expression of RhoA protein and mRNA in HUVECs were detected by Western blotting and semi-quantitative RT-PCR.Results Thrombin or LPS induced F-actin depolymerized and significant increase in the permeability of HUVECs.Permeability and F-actin do not change significantly when Y-27632 was added.Thrombin or LPS could upregulate the expression of RhoA protein and mRNA in HUVECs.Conclusion HUVECs monolayer permeability injury induced by thrombin or LPS is related to depolymerization of F-actin and RhoA signal pathway is involved.
