Effects of The Modification of Heparin 6-Carboxyl Group on Inhibitive Activity of P-Selectin-mediated A375 Cells Adhesion
- VernacularTitle:肝素6位羧基修饰对抑制P-选择素介导的A375细胞粘附活性的影响
- Author:
Min WEI
;
Meihong TIAN
;
Lin CHEN
;
Xianlu ZENG
- Publication Type:Journal Article
- Keywords:
heparin, carboxyl-reduced heparin, P-selectin, platelet, tumor cell adhesion
- From:
Progress in Biochemistry and Biophysics
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Several studies have demonstrated that heparin can significantly inhibit the P-selectin-mediated interaction of platelets and tumor cells during metastasis as a P-selectin ligand. However, little information is available about the specific oligosaccharide structures of heparin in recognition by P-selectin. Two chemically modified heparins, CR-heparin and SCR-heparin were prepared, to explore if such heparin derivatives can reduce the P-selectin-mediated A375 tumor cell adhesion. The results indicated that CR-heparin with low anticoagulant activity could significantly inhibit the P-selectin-mediated A375 tumor cell adhesion, demonstrating that 6-carboxyl group of the glucuronic acid in heparin may not be crucial for recognizing by P-selectin. In contrast, SCR-heparin reduced the inhibiting activity dramatically, suggesting that the recognition of P-selectin to heparin depend on not only densities of negative charge. These results provide valuable experimental evidence for clarifying the molecular mechanism of P-selectin-mediated tumor cell adhesion.
