Transdifferentiation of mesenchymal stem cells isolated from the rabbit mobilized peripheral blood into cardiomyocytes in vitro
- VernacularTitle:动员后兔外周血间充质干细胞体外诱导转分化为心肌细胞
- Author:
Hongwei LIU
;
Luyue GAI
;
Duanzhen ZHANG
- Publication Type:Journal Article
- Keywords:
peripheral blood;
mesenchymal stem cells;
cardiomyocytes
- From:
Basic & Clinical Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate mesenchymal stem cells(MSCs) from the mobilized peripheral blood and transdifferente them into cardiomyocytes for establishing an non-invasive cell source of stem cell transplantation for myocardial infarction therapy.Methods To granulocyte-colony stimulating factor((30 ?g/kg?day)) was injected into New Zealand White rabbits subcutaneously for 5 days,then MSCs were isolated from peripheral blood.After treatment with 5-azacytidine for 24 hours,the cultured cells were evaluated by immunohistochemical staining for actin,desmin and cardiac-specific troponin T(cTNT).Results After treatment with 5-azacytidine,the MSCs from the mobilized peripheral blood showed spindle-like and multinucleation,some cells aggregated and formed a ball-like appearance at 2 weeks.The ball-like cells and some spindle-like multinuclear cells were found with positively staining of actin,desmin and cTNT.Conclusion MSCs can be isolated from the mobilized peripheral blood and transdifferented into cardiomyocytes in vitro,suggesting that MSCs from the mobilized peripheral blood may be a new and non-invasive cell source of stem cell transplantation in myocardial infarction therapy.
