Arsenic trioxide inhibits proliferation and promotes apoptosis of rat vascular smooth muscle cells
- VernacularTitle:三氧化二砷抑制大鼠血管平滑肌细胞增殖并促进细胞凋亡
- Author:
Zhuoqi ZHANG
- Publication Type:Journal Article
- Keywords:
smooth muscle cells;
vascular;
arsenic trioxide;
proliferation;
apoptosis
- From:
Basic & Clinical Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the anti-proliferation,pro-apoptosis and cell cycle blocking effects of arsenic trioxide(As_(2)O_(3)) on rat vascular smooth muscle cells(VSMCs).Methods The effects of As_(2)O_(3) on VSMCs viability,growth and proliferation were assayed by MTT,trypan blue exclusion and()~3H-thymidine incorporation methods.Change of cell cycle and apoptosis of VSMCs induced by As_(2)O_(3) were observed by flow cytometry and DNA laddering methods.Western blot was applied to detect the expression changes of P53 and P21()~(waf1/cip1) proteins.As_(2)O_(3) inhibited the growth and DNA synthesis of VSMCs both time-and dose-dependently,while had no obvious cytotoxic effects on cell viability at the concentrations from 1 to(16 ?mol/L).(8 ?mol/L) of As_(2)O_(3) significantly blocked the cell cycle progression,decreased the S phase and increased G_(0)G_(1) phase partition with sub-G_(1) apoptotic distribution. Results Typical DNA fragmentation of VSMCs induced by(16 ?mol/L) of As_(2)O_(3) was observed at different time points.(8 ?mol/L) of As_(2)O_(3) significantly up-regulated P53and P21()~(wif1/cip1) expression in a time-dependent manner.Conclusion These results suggest that As_(2)O_(3) inhibited the proliferation,accelerated the apoptosis and blocked the cell cycle progression in VSMCs which may relate to P53 and P21~(waf1/cip1) pathway.
