Protective effect of Poly gala tenui folia willd on genetic damage and enhancement of lymphocyte function induced by cyclophosphamide in mice
- VernacularTitle:中草药远志对环磷酰胺所致小鼠遗传物质损伤的保护作用和淋巴细胞功能的增强作用
- Author:
Dezhong WEN
;
Heyan ZHANG
;
Yuzhuo ZHU
;
Lijiao ZHANG
- Publication Type:Journal Article
- Keywords:
Polygala tenuifolia/pharmacology;
cyclophosphamide;
lymphocyte proliferation;
suppression, genetic
- From:
Journal of Jilin University(Medicine Edition)
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of anti-mutation of Chinese medicinal herb Poly gala tenui folia willd on T lymphocyte proliferation in spleen. Methods The micronucleus test of mouse bone marrow cell (MNT): thirty mice were divided into six groups (n = 5) , negative control (NS) , cyclophosphamide group (CP 3. 0 mg ? kg-1) , Poly gala tenui folia willd antimutagenesis groups (Polygala tenui folia willd with dosage of 0.5, 1.0. 2.0, 4.0 g ? kg-1 + CP 30 mg ? kg-1 ). The improved method was used to detect the micro-nuclei frequency. Lymphocyte transformation test: twenty-four mice were divided into four groups (n = 6), saline control , CP control (3.0 mg ? kg-1), Polygala tenui folia willd (2.0 g ? kg-1), Polygala tenui folia willd + CP (2. 0 g ? kg-1 Polygala tenuifolia willd + CP 30 mg ? kg- ) group. MTT assay was used to calculate the stimulation index (SI). Results The micro-nuclei frequency was significant difference between Poly gala tenui folia willd antimutagenesis groups and CP groups (P
