The quality control and standardization of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry
- VernacularTitle:表面增强激光解析电离飞行时间质谱技术的质量控制与标准化
- Author:
Yanying ZHENG
;
Yajie WANG
;
Xixiong KANG
- Publication Type:Journal Article
- Keywords:
Proteomics;
Standardization;
Surface-enhanced Laser Desorption/ionization Time-of-Flight Mass Spectrometry
- From:
Chinese Journal of Laboratory Medicine
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
As the advanced technology of proteomics, the surface-enhanced laser desorption/ionization Time-of-Flight Mass Spectrometry(SELDI-TOF-MS)has great potential in clinical application for it′s technological features.Whether it can be applied in clinical trials depends on the quality control and standardization of the technology.In the article, we introduced and discussed the following aspects of the issue.1) quality controlling before sample being analyzed, including the collection and preparation of samples, the selection and conservation of reagents, the calibration and attendance of laboratory apparatus;2) quality controlling during the assay, that is, the validation and analysis of the experimental result;3) the standardization of the continued data handling and analyzing, including the standardization of the analyzed data′s nature, the selection and standardization of the data′s analyzing methods, the system evaluation of the whole SELDI-TOF-MS technological system.
