Effect of oxidized low density lipoprotein on CD36 expression in macrophage
- VernacularTitle:氧化低密度脂蛋白对THP-1巨噬细胞CD36表达的影响
- Author:
Zhongcheng MO
- Publication Type:Journal Article
- Keywords:
CD36;
THP-1 macrophage;
Oxidized low density lipoprotein;
Cholesterol influx;
Atherosclerosis
- From:
Journal of Medical Postgraduates
2004;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of oxLDL on CD36 expression and cholesterol influx in THP-1 macrophage. Methods: THP1 macrophage were treated with 50mg/L oxLDL for 0,12,24,36,48 h respectively.Oil red O staining was used to observe the intracellular lipid droplets.\labeled Cholesterol influx was determined by FJ-2107P type liquid scintillator.CD36 mRNA and protein level were determined by reverse transcription-polymerase chain reaction(RT-PCR) and Western blotting respectively. Results:We found that oxLDL elevated CD36 in both protein and mRNA levels and increased cholesterol influx in a time-dependent manner.The levels of cholesterol influx were 23.5%、(27.8%、)39.7%、44.1% and 49.7% respectively. Conclusion: oxLDL may upregulate CD36 expression and increase cholesterol influx.
