Effects of Astragalus on the apoptosis of cultured human peritoneal mesothelium induced by peritoneal dialysis solution
- VernacularTitle:黄芪注射液拮抗腹膜透析液诱导腹膜间皮细胞凋亡的研究
- Author:
Miao ZHANG
;
Chunming JIANG
;
Zheng SUN
- Publication Type:Journal Article
- Keywords:
Astragalus;
Peritoneal dialysis;
Mesothelial cell;
Apoptosis
- From:
Journal of Medical Postgraduates
2004;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Astragalus on the the apoptosis of cultured human peritoneal mesothelium induced by peritoneal dialysis solution(PDS). Methods:Using RPM1640 culture medium as control,PDS group(commercial PDS containing 1.5% glucose without Astragalus),Astragalus group 1(commercial PDS containing 1.5% glucose and 1% Astragalus) and Astragalus group 2(commercial PDS containing 1.5% glucose and 2% Astragalus) were tested to explore the apoptosis of cultured human peritoneal mesothelial cell in each group.The activity of caspase-3,the terminal deoxynucleotidyl transferase(TdT) mediated dUTP nick end labeling(TUNEL) and flow cytometry analysis were used in apoptosis analysis in this study. Results: The activity of caspase-3 in the control group was defined as 1,the relative activity of caspase-3 in PDS group、 Astragalus group 1 and Astragalus group 2 were 3.26?0.91,(1.87?)0.43 and 1.67?0.32 respectively.The activity of caspase-3 in PDS group was significantly higher than those in the Astragalus group 1 and Astragalus group 2(P
