Isolation and identification of human anti-oxidized LDL autoantibodies
- VernacularTitle:人血清氧化低密度脂蛋白自身抗体的提取和初步鉴定
- Author:
Junjun WANG
;
Yanfei ZHU
;
Hongjuan QIANG
;
Chunni ZHANG
- Publication Type:Journal Article
- Keywords:
low density lipoprotein;
oxidation;
autoantibody;
atheroscloresis.
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate and identify human anti-oxidized low density lipoprotein (LDL) autoantibodies.Methods Oxidized LDL (Ox-LDL) autoantibodies were isolated from eight healthy subjects by affinity chromatography with using an Ox-LDL cross-linked Sepharose 4B column.Results Three elution peaks of Ox-LDL autoantibodies were washed out from the Ox-LDL column by NaHCO3,acetate and KCNS buffer in turn and collected respectively.Ox-LDL autoantibodies were isolated from all the selected healthy subjects.All the three elution peaks had good reactivity with Ox-LDL.Among them,the elution peaks by KCNS buffer had high protein level and strong reactivity with Ox-LDL.The types of these antibodies included IgG and IgM, and IgG was the main type.Conclusion The isolated Ox-LDL autoantibodies had strong reactivity with Ox-LDL.
