Quantitative Real-time Detection of Spontaneous Perforin(PFR) Gene Expression in Peripheral Blood Mononuclear Cell(PBMC) by Polymerase Chain Reaction
- VernacularTitle:生理状态下外周血白细胞穿孔素基因表达的荧光定量PCR检测
- Author:
Ru WANG
;
Peijie CHEN
;
Qianggang DONG
- Publication Type:Journal Article
- Keywords:
perforin(PFR),mRNA,real-time PCR
- From:
Chinese Journal of Sports Medicine
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method for quantitative real-time detection of perforin(PFR) using polymerase chain reaction(PCR).Methods Gene expression of PFR in peripheral blood mononuclear cell(PBMC) from 25 healthy individuals was detected by PCR.An endogenous"housekeeping" gene GAPDH was used to normalize the results.Results and Conclusion The spontaneous PFR mRNA expression in PBMC in a given sample could be measured by PCR with relativity correlation coefficient of r=1.
