Modification of the Full-length cDNA Clone of Newcastle Disease Virus of Goose Origin~*
- VernacularTitle:鹅源新城疫病毒ZJI株基因组cDNA克隆的序列修饰
- Author:
Yuliang LIU
- Publication Type:Journal Article
- Keywords:
Newcastle disease virus of goose origin,Site-directed mutagenesis,Genomic cDNA clone,Modification
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
The 6.5kb specific fragment containing the T7 promoter and the transcription vector was cut down from the full-length cDNA clone of Newcastle disease virus strain ZJI of goose origin,and thereafter it was self-ligated to form the high guality plasmid for mutagenesis.Site-directed mutagenesis technique was used for inserting three additional G nucleotides(nts) into the region between the T7 promter and the leader sequence of the NDV.The RT-PCR was employed to amplify the F/HN genes fragements,and then they were ligated by the shairing restriction enzyme BsmBI and finally the corresponding fragment in the mutatant full-length cDNA was substituted by the new one.The sequencing results showed that the three additional G nts were successfully inserted and the mutant nts in the full-length cDNA were corrected and all these studies lay a foundation for the research on the reverse genetics of NDV strain ZJI.
