Studies of 4500 Umbilical Cord Blood Units Banking
- VernacularTitle:4500份脐带血造血干细胞保存研究
- Author:
Jimling HAN
- Publication Type:Journal Article
- Keywords:
hematopoietic stem cells;
umbilical cord blood;
hematopoietic stem cell transplantation;
standard operation procedures (SOP)
- From:
Chinese Journal of Cancer Biotherapy
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare and cryopreserve umbilical cord blood (UCB) units for clinical hematopoietic stem cell transplantation. Methods; HSC of UCB units that met our standards were collected, separated cryopreserved and tested. Results: 4 500 (72. 2%) of 6 232 cord blood units were suitable for preservation. Average net volume, numbers of total nucleated cells, percentage of CD34~+ cells and CFU-C of the UCB units were 90. 7?22. 2 ml, 10. 5?3. 4?10~8, 0. 24?0. 15%, 78.9?44. 14/10~5 NC, respectively. After separation, the cell recovery of nucleated cells, CD34~+ cells and CFU-C were 82. 1?6. 52%, 92. 5?6. 15% and 90?9. 67%, respectively. The cell viability after separating was over 95%. After thawing, the recovery of nucleated cells, CD34~+ cells and CFU-C were 84. 2?10. 1%, 96. 5?21. 8% and 115. 1?23. 3%, respectively. The cell viability was 93. 8?4. 4%. Among the 569 searching cases,532 (93%) cases could found 4/6 of HLA-A, B and DR loci matched UCB units in our bank, including 39 (6. 58%) found 6 loci matched and 237 (41. 65%) found 5 loci matched. Conclusion: These results indicated that we can supply high quality cord blood HSC for clinical transplantation.
