Measurement of cardiac vascular endothelial growth factor gradients in patients with coronary artery disease and its clinical significance
- VernacularTitle:冠心病患者心局部血管内皮生长因子的检测及临床意义
- Author:
Bin CUI
;
Lan HUANG
;
Yaoming SONG
- Publication Type:Journal Article
- Keywords:
Coronary disease;
Endothelial growth factors;
Endothelial progenitor cells
- From:
Chinese Journal of Interventional Cardiology
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the serum level of vascular endothelial growth factor (VEGF) in patients with coronary artery disease (CAD) and its clinical significance. Methods The study included 36 patients with CAD and 10 non-coronary heart disease patients as the control. The concentrations of serum VEGF in the coronary veins and the aorta were measured by an enzyme-linked immunsorbent assay (ELISA). Total mononuclear cells were isolated from peripheral blood by Ficoll density gradient centrifugation, and were cultured in M199 medium supplemented with 20% fetal bovine serum. After 7 days culture, attached cells were collected. The effect of VEGF on the migration of endothelial progenitor cells (EPCs) were assayed with modified Boyden chamber assay. Results The serum VEGF levels of stable angina pectoris (SAP) patients (208.46?44.74 ?g/L) and acute coronary syndrome (ACS) patients (267.29?93.99 ?g/L) were much higher than that of the controls group (120.77?26.25 ?g/L,P
