Detection and Identification of Tilletia controversa K?hn by Real-time fluorescent PCR
- VernacularTitle:实时荧光PCR技术对小麦矮腥黑穗病菌的检测
- Author:
Qiang GAO
- Publication Type:Journal Article
- Keywords:
TCK,TCT,TFL,ETS,Real-time fluorescent PCR
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
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Abstract:
By analysing the ribosome gene(rDNA) sequences in externally transcribed spacer(ETS) of wheat T.controversa(TCK) and its similar spacies T.caries(TCT)and T.foetida(TFL).The special sequences of TCK's ETS have been found,.And designed Taqman probe according to the special sequences,TCK has been successfully detected by using Real-time Fluorescent PCR.