Improvement of Ethanol Fermentation by Expression of Schizosaccharomyces pombe mel Gene and Disruption of GPD1 in Saccharomyces cerevisiae
- VernacularTitle:在GPD1中整合表达蜜二糖酶基因改善酒精发酵水平
- Author:
Guiyang SHI
- Publication Type:Journal Article
- Keywords:
Melibiose,?-galactosidase,Ethanol fermentation
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
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Abstract:
Based on homologous recombination,?-galactosidase gene mel from S.pombe was integrated to the chromosomal DNA of industrial S.cerevisiae and the key enzyme gene GPD1 of the glycerol anabolic pathway was disrupted contemporaneously,and recombinants were screened through increasing G418 concentration.It was shown that melibiose utility capability of recombinant S.cerevisiae MG1 was increased evidently,glycerol productivity was decreased,and the mel gene was expressed stabilized in the host cell.No impact on the cell character was appeared after introduced extrinsic gene,but the cells were flocculate when growing.When compared with parent industrial S. cerevisiae Y to ferment with corn powder and wheat starch as raw substrates,ethanol productivity was increased,glycerol productivity was decreased,and the melibiose in the broth was exhausted completely detected by HPLC analysis.
