Expression of nNOS mRNA of brain tissue at early stage of intracerebral hemorrhage in rats and the intervention effect of Didangtang
- VernacularTitle:大鼠脑出血早期脑组织神经元型一氧化氮合酶mRNA表达及中药抵当汤干预对其的影响
- Author:
Shaohua LIU
;
Haoxin WU
;
Yajun JIANG
- Publication Type:Journal Article
- Keywords:
intracerebral hemorrhage;
nNOS;
hybridization in situ;
Didangtang
- From:
Journal of Clinical Neurology
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of neuron nitric oxide synthase (nNOS) mRNA of brain tissue at early stage of intracerebral hemorrhage (ICH) in rats and the intervention effect of Didangtang.Methods 72 male Sprague-Dawley rats were randomly divided into four groups: intracerebral hemorrhage group, Didangtang treatment group, NOS inhibition group and normal saline group. Quantitate fresh autologous blood was infused into right caudate nucleus of rat sterotaxically to build up experimental ICH model and normal saline was instead in control group. The neurological function deficit scores were observed by Bederson method (3 grades) at 6 h, 24 h and 72 h after operation. At the same time points, the rats were sacrificed and the brain tissues were taken out for the measurement of nNOS mRNA by technique of hybridization in situ.Results Neurological function deficit scores of the rats were significantly improved both in intracerebral hemorrhage group and Didangtang treatment group at 72 h after operation(all P
