Effects of EPO on caspase-9 mRNA expression after globe ischemia-reperfusion in rats
- VernacularTitle:促红细胞生成素对大鼠脑缺血再灌注后半胱氨酸天冬酶-9 mRNA表达的影响
- Author:
Xiaoying LIN
;
Tao HUANG
;
Zhongxin LI
- Publication Type:Journal Article
- Keywords:
caspase-9;
EPO;
ischemia-reperfusion;
RT-PCR
- From:
Journal of Clinical Neurology
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of erythropoietin (EPO) on caspase-9 mRNA expression after globe ischemia-reperfusion in rats.Methods The globe ischemia-reperfusion models of rats were divided randomly into three groups: group A, group B and group C. Both group A and group B included 5 subgroups according to different reperfusion time (6 h, 12 h, 24 h, 48 h and 72 h). The rats of group B were treated with EPO after globe ischemia-reperfusion. Brain tissues were taken out after execution at different time point. The expression of caspase-9 mRNA was detected with reverse transcription PCR (RT-PCR) technique.Results (1) After ischemia-reperfusion, the expressions of caspase-9 mRNA in group A were significantly higher than those in group B and group C (all P
