Protective effect of minocycline on cell apoptotic model of Parkinson's disease
- VernacularTitle:美满霉素对帕金森病细胞凋亡模型保护作用的研究
- Author:
Wei SHEN
;
Shenggang SUN
;
Xuebing CAO
- Publication Type:Journal Article
- Keywords:
minocycline;
PC12 cell;
apoptosis;
Parkinson disease's;
caspase-3
- From:
Journal of Clinical Neurology
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effect mechanism of minocycline (MC) on cell apoptotic model of Parkinson's disease induced by 1-methyl-4-phenylpyridinium (MPP+ ).Methods Different concentrations of MPP+ (10, 50, 250, 500 ?mol/L) were added into the culture of PC12 cells. The most appropriate concentration of MPP+ (MPP+ group) was selected to establish apoptotic model of dopaminergic neurons. For selecting the most a-ppropriate concentration MC (MC+MPP+ group) of protective effect, MTT was used to assay the viability of model of dopaminergic neurons pretreated by different concentrations MC (0, 10, 50, 100, 200 ?mol/L). The cell apoptosis, apoptosis ratio and caspase-3 mRNA expression of MPP+ group and MC+MPP+ group were assayed by electrophoresis method, flow cytometry and RT-PCR, and compared with control group.Results (1) At concentration of 10 ?mol/L of MPP+, cell parkinsonism model of apoptosis was established. The cell viability of apoptotic model of dopaminergic neurons was the highest when pretreated by 100 ?mol/L of MC ( P
