Evaluation of chemosensitivity of human colorectal cancer determined by APT-TCA assay in vitro
- VernacularTitle:人大肠癌原代培养体外药敏试验的研究
- Author:
Yanfen LI
;
Haimei TIAN
;
Zhixiang ZHOU
;
Dongyan CAO
;
Jianxiong WU
;
Weiqi RONG
;
Yi LIU
;
Mo LI
;
Haipeng WANG
;
Xiaobing WANG
;
Zhaoyang LIU
;
Zhi LIANG
;
Dongwan CHENG
;
Wei ZHANG
- Publication Type:Journal Article
- Keywords:
Ttumor chemosensitivity assay;
Colorectal cancer;
Chemosensitivity
- From:
Chinese Journal of Laboratory Medicine
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the heterogeneity of chemosensitivity in colorectal cancer using an ATP-tumor chemosensitivity assay (ATP-TCA) and the feasibility of individual chemotherapy.Methods An ATP-TCA were used to determine the effect of 16 single or combined cytotoxic drugs in surgical specimens from 50 patients with colorectal cancer.Results There were considerable differences in chemosensitivity between individuals.The most active single drugs in the assay was identified as Navelbine, Hydroxycamptothecin, 5-Fluorouracil and Paclitaxel; 34.1%, 31.6%, 27.6% and 24.3% of specimens showed sensitivity to them, respectively.5-Fluorouracil+Mitomycin+Aytarabine was found to be the most effective combination, for 100% (11/11)specimens were sensitive to this regimen.5-Fluorouracil+Cisplatin+Adriamycin and Gemcitabine+Cisplatin were moderately active regimens.Conclusions There was the heterogeneity of the in vitro chemosensitivity in colorectal cancer.The use of the ATP-TCA provides a method of selecting appropriate anti-cancer drugs in colorectal cancer.
