Expression level and clinical significance of cyclinB1 in primary hepatocellular carcinoma
- VernacularTitle:原发性肝癌组织cyclinB1表达水平及其临床诊断意义
- Author:
Huochun YI
;
Ruxin HUANG
;
Zhongying ZHANG
- Publication Type:Journal Article
- Keywords:
Liver neoplasms;
Cyclin B;
Reverse transcriptase-polymerase chain reaction
- From:
Chinese Journal of Laboratory Medicine
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression level and clinical significance of cyclinB1 in primary hepatocellular carcinoma(PHC).Methods Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the mRNA level of cyclinB1 in liver cancer tissues and adjacent tissues of cancer.Results CyclinB1 mRNA was positive expression in 90%(36/40)cancer tissues and 85%(34/40)adjacent tissues of cancer.The expression level of cyclinB1 in cancer tissues (0.531?0.015)was significantly higher than that in adjacent tissues of cancer(0.263?0.023).The level of cyclinB1 was significantly associated with pathological grades and lymph node metastasis, but not associated with sex、age、tumor size or tumor thrombus.Conclusion CyclinB1 may play an important role in the tumorigenesis of PHC and may be a potential adjuvant parameter in evaluating pathological grades and lymph node metastasis.
