A sensitive and specific method for detection of nuclear transcription factor C/EBP? based on the complexes of DNA-binding-C/EBP?
- VernacularTitle:利用DNA结合活性检测核转录因子C/EBP?方法的建立
- Author:
Jianrong SU
;
Zheng ZHANG
;
Dongxu SUN
- Publication Type:Journal Article
- Keywords:
Transcription factor;
Electrophoresis
- From:
Chinese Journal of Laboratory Medicine
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a perfect method that is based on the complexes of DNA-binding-C/EBP? for detection of nuclear transcription factor C/EBP? (CCAAT enhancer binding protein ?). Methods To search specific oligonucleotides sequence and establish a labeling method of consensus oligonucleotides and the standard curve was regressed.The electrophoresis conditions were optimized.Results The combination of 20pmol of ?- 32 P-ATP and 4 pmol of C/EBP? oligonuleotides in reaction can express the highest probe activity and corporation effect.The linearity was established (r2=0.975). 0.5?g of nuclear extract can be detected and bands are clear and specific.Conclusion The method is good at specificity. It can be a method to detecting nuclear factor C/EBP? or its DNA-binding site.
