Efficient measurement of platelet-monocyte aggregates in whole blood by flow cytometry
- VernacularTitle:流式细胞术检测血小板-单核细胞聚集的影响因素
- Author:
Junhong WANG
;
Guangming QIN
;
Songzhao ZHANG
;
Yaping JIN
- Publication Type:Journal Article
- Keywords:
Platelet aggregation;
Flow cytometry;
Monocytes
- From:
Chinese Journal of Laboratory Medicine
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of the measurement of the platelet-monocyte aggregates (PMAs) by using of flow cytometry (FCM).Methods Anticoagulated peripheral venous bloods from nine healthy donors were incubated with a PE-CD14 MAb (monocyte marker) and a FITC-CD42a MAb (platelet marker) for 20 min and the formations of PMAs were measured by use of FCM.The factors such as fixative, anticoagulant, storage time and temperature were analyzed.Results The PMAs of citrated whole blood increased with the time elapsed in 6 h after blood drawing when they were stored in the room temperature.The PMAs of each time point showed significant difference (P
