The effects of preconditioning with lithium on the expressions of heat shock protein 70 in gerbil after global ischemia
- VernacularTitle:锂对沙土鼠脑缺血后热休克蛋白70表达的影响
- Author:
Tao SHI
;
Yanning QIAN
;
Bin WANG
- Publication Type:Journal Article
- Keywords:
Brain ischemia;
Precondition;
Lithium;
Delayed neuron death;
Heat shock protein 70
- From:
Journal of Medical Postgraduates
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of preconditioning with lithium on the expressions of HSP70 in gerbil after global ischemia. Methods: The transient ischemia model of gerbil global was established by clamping the bilateral common carotid arteries for 5 min.36 gerbils were randomly divided into group lithium(n=18) and group control(n=18).Gerbils in group lithium were injected with lithium intraperitoneally,5 mmol/kg,once a day for 5 consecutive days.Normal saline was used instead of lithium in group control as vehicle control.Both group lithium and control underwent ischemia after preconditioning,and were then further divided into three subgroups(n=6,in each subgroup).The garbles in lithium 1 or control 1 subgroup were sacrificed 1 day after ischemi;lithium 3 or control 3 sacrificed in 3 days;and lithium 7 or control 7 were sacrificed in 7 days.The brains were made into paraffin sections and were used for immunohistochemistry against HSP70. Results: Compared with the vehicle control,the HSP70 expression in subgroup lithium1 was inhibited in the hippocampus CA1(P
