Preliminary studies of prevention of interleukin-6 antisense oligonucleotide liposomes on after cataract in rabbit
- VernacularTitle:白细胞介素-6反义寡核苷酸脂质体预防后发性白内障的初步研究
- Author:
Xiaoyan DENG
;
Xiaojun CAI
;
Zhisheng KE
- Publication Type:Journal Article
- Keywords:
Interleukin-6;
Oligonucleotide,antisense;
Liposomes;
Cataract,after
- From:
Journal of Medical Postgraduates
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the safety and efficacy of interleukin-6 antisense oligonucleotide liposomes on the experimental animals for prevention of after cataract. Methods:Thirty healthy New-Zealand rabbits(30 left eyes) received extracapsular lens extraction,and were randomly divided into 3 groups: anterior chamber injection of 0.1ml of interleukin-6 antisense oligonucleotide liposomes(Group A),blank liposomes(Group B) after operation respectively;and control group(Group C).All left eyes were examined by slit-lamp microscope and ophthalmoscope regularly.The aqueous humor IL-6 concentrations were measured 1 day before operation,and 1 day,3 days,7 days,14 days and 30 days after operation through enzyme-linked immunoabsorbent assay.Three months later,photographs of the posterior lens capsules were taken through digital slit-lamp microscope and the histopathological changes of posterior lens capsules were observed by light microscope. Results:①Three months after the operation,the number of eyes developing posterior capsule opacification in Group A,B and C were 7,10 and 10 respectively,with significant difference(P=0.037).② The average value of Group A was lower than Group B and C,with statistically significant difference(P
