The significance of intercellular adhesion molecule-1 and tumor necrosis factor-? in experiment of myocardium hypertrophy
- VernacularTitle:细胞间黏附分子-1和肿瘤坏死因子?在心肌肥厚实验中的意义
- Author:
Xiao TAN
- Publication Type:Journal Article
- Keywords:
Intercellular adhesion molecule-1;
Tumor necrosis factor-?;
Myocardial hypertrophy;
Angiotesin-converting enzyme inhibitor
- From:
Journal of Medical Postgraduates
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:①To study the expression of Intercellular adhesion molecule-1(ICAM-1)、Tumor necrosis factor-?(TNF-?)in the hypertrophic myocardium of rat from the level of transcription and translation and the expression of ICAM-1、TNF-? in the normal myocardium of rat,and compare them,find the law of expression,inquire into relation between myocardial hypertrophy and ICAM-1、TNF-?,and to explain myocardial hypertrophy's pathogenesis preliminarily.②To observe above items after using ACEI and inquire into pathogenesis of ACEI in reversing myocardial hypertrophy.Methods:30 SD rats were divided into 3 groups and one group contained 10 rats.The first group was myocardial hypertrophic group.The second was myocardial hypertrophic group that was treated with ACEI.The thirth was control.After 8 weeks,they were killed and cardiae specimen were obtained.The proteins of ICAM-1、TNF-? were detected by immunohistochemistry and(LV's) specimen were detected in ICAM1(mRNA、)(TNF-?)(mRNA) with RT-PCR.Results: The expression of proteins of ICAM-1、(TNF-?) in the first group increased and dropped in the second group.That of the third is the lowest.The expression of ICAM-1 (mRNA、)(TNF-?) mRNA was the same to above. Conclusion:ICAM1、TNF-? plays a role in myocardial hypertrophy pathogenesis.ACEI which lowers the expression of ICAM-1、TNF-? can results in reversing myocardial hypertrophy.
