Immunohistochemical Study for MAPK Expression in UV Irradiated Melanocytic Nevi.
- Author:
Mann Kyu PARK
1
;
Kyu Cherl CHOI
;
Byoung Soo CHUNG
Author Information
1. Department of Dermatology, Chosun University Medical School, Gwangju, Korea. bsjung@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
Mitogen-activated protein kinase;
ERK;
JNK;
p38;
UVB;
Melanocytic nevi
- MeSH:
Antibodies, Monoclonal;
Apoptosis;
Carcinogenesis;
Cytokines;
Cytoplasm;
Healthy Volunteers;
Humans;
Immunohistochemistry;
Intercellular Signaling Peptides and Proteins;
Nevus;
Nevus, Pigmented*;
Phosphotransferases;
Protein Kinases;
Skin Neoplasms;
Ultraviolet Rays
- From:Korean Journal of Dermatology
2005;43(9):1186-1191
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: In contrast to the relationship between UV exposure and nonmelanoma skin cancers, molecular evidence for the involvement of UV radiation in melanocytic tumorigenesis is quite limited. Mitogen-activated protein kinase (MAPK) is an important molecule in transducing extracellular signals from the cell surface to the nucleus. The MAPK family includes ERK (extracellular signal- regulated protein kinase), JNK (stress-activated c-Jun N-terminal kinase), and p38 kinases. Various growth factors and cytokines, as well as other signals such as UV light are able to activate MAPK, resulting in a variety of cellular responses including proliferation, differentiation and apoptosis. OBJECTIVE: The purpose of our study was to investigate the effect of UV irradiation on the expression of MAPK in melanocytic nevi. METHOD: Melanocytic nevi from ten healthy volunteers were partially covered, irradiated with a defined UV dose, and excised after 1 week. healthy The irradiated and non-irradiated area were examined separateted by immunohistochemistry using p-ERK, p-JNK, and p-p38 monoclonal antibodies. RESULTS: In the non-irradiated area of melanocytic nevus, p-ERK, p-JNK, and p-p38 were undetectable in nevus cells. After irradiation, p-ERK expression was observed in nevus cells in 7 cases, and p-p38 was stained diffusely in the cytoplasm of nevus cells in 6 cases, but there was no immunoreactivity of p-JNK. CONCLUSION: We suggest that a single UV irradiation of melanocytic nevi can stimulate both p-ERK and cytoplasmic p-p38 expression, but not p-JNK.
