Regulation of proliferation and protein expression in GTN treated tumor cells
- VernacularTitle:硝酸甘油对肿瘤细胞增殖及蛋白表达的调控
- Author:
Hui JIANG
;
Yaping TIAN
;
Jin DONG
;
Guoqing ZHANG
- Publication Type:Journal Article
- Keywords:
Nitroglycerin;
Proteomics;
Cervix neoplasms Hela cells
- From:
Chinese Journal of Laboratory Medicine
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the proliferation and analyze the protein expression of the Hela cells by the regulation of glyceryl trinitrate (GTN) that acts as the donor of nitric oxide.Methods Cell morphology and MTT assay were used to analyze cell proliferation.Two-dimensional polyacrylamide gel electrophoresis (2-DE) and computer-assisted image analysis find the spots of interests. Difference of protein expression between GTN-treating group and control group was detected.Results Hela cells treated with 40?g/ml GTN in culture medium underwent inhibition of the growth activity, which were not clustered and shriveled. Some atypia cells were observed.Density of the cells was obviously decreased(0.0825, P
