Effects of PTEN gene transfection on apoptosis of human bladder cancer cell lines BIU-87
- VernacularTitle:PTEN基因转染对人膀胱癌细胞BIU-87凋亡的影响
- Author:
Jianhua ZHANG
;
Weimin YANG
;
Siwei ZHOU
- Publication Type:Journal Article
- Keywords:
PTEN gene;
Transfection;
Bladder cancer;
Gene therapy
- From:
Journal of Medical Postgraduates
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of anti-oncogene PTEN transfection on apoptosis of human bladder cancer cell lines,BIU-87,and to bring a new method of gene therapy for bladder cancer. Methods:A eukaryotic expression vector containing PTEN was transfected into BIU-87,and positive cell clones were selected and amplified.Expression of PTEN was detected by RT-PCR.Apoptosis of BIU-87 were measured before and after transfection by in situ cell apoptosis detection kit and flow cytometry. Results:PTEN transfected BIU-87 could steadily express PTEN mRNA,and cell apoptosis significantly increased compared with control groups. Conclusion:Transfection of PTEN might induce apoptosis of bladder cancer cells so as to inhibit the occurrence and development of tumor.
