Protective effect of ischemic preconditioning in the posterior limbs of rats on ischemic reperfusion injury of donor pancreas graft
- VernacularTitle:大鼠双后肢缺血预处理对移植胰缺血-再灌注损伤的保护作用
- Author:
Xiaonan LIU
- Publication Type:Journal Article
- Keywords:
Pancreas transplantation alone;
Ischemic preconditioning;
Ischemic reperfusion;
Rat
- From:
Journal of Medical Postgraduates
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of ischemic preconditioning(IPC) in the posterior limbs of rats on ischemic reperfusion(I/R) injury of donor pancreas graft,and to analyze the possible mechanism.Methods: With Group Control(n=6,normal) suffered with sham operation,36 streptozocin(STZ)-induced diabetic SD rats were randomly assigned to 6 groups: Group I/R(n=6) received pancreas transplantation alone(PTA),Group IPC(n=6) received pancreas transplantation alone(PTA) plus IPC,which included three cyclings of 5 minutes ischemic and 5 minutes reperfusion before reperfusing donor pancreas,Group IPC+L-NAME(n=6),Group IPC+DPCPX,Group IPC+Nal.and Group IPC+AA received the same treatment with Group IPC,but separately injected NOS antagonist L-arginine methyl ester(L-NAME),adenosine A1 receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine(DPCPX),opioid antagonist naloxone and oxygen radical scavenger ascorbic acid before IPC.The blood glucose was monitored before and after reperfusion,the TNF-? in serum,MDA and MPO in pancreatic tissue were monitored after reperfusion,and apoptotic cells were stained by TUNEL technique 2 h after reperfusion. Results: ①The blood glucose,serum TNF-?,and pancreas MDA and MPO of group(I/R,) Group IPC+L-NAME,Group(IPC+)Nal.and Group IPC+AA were higher than those of Group IPC after reperfusion(P
