Effects of mutation induced by microwave irradiation on antifungal activity of marine strain B1
- VernacularTitle:利用微波诱变技术提高菌株B1抑菌活性的研究
- Author:
Jiuming ZHANG
- Publication Type:Journal Article
- Keywords:
marine strain;
microwave irradiation;
mutation parameter;
heredity stability;
induced mutation
- From:
Chinese Journal of Marine Drugs
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of microwave irradiation on a marine mutant strain B1 which had high antifungal activity,and obtain the optimum dosage of microwave irradiation.Methods The marine strain B1(Bacillus sp.) was radiated by 2450 MHz microwave for various times.The mutation rate p,mutation mean ? and the mutation variance ?~(2) were estimated by statistical methods.From these mutants,a high-activity strain B1-413 was obtained,and its genetic attribute was tested.Results Microwave irradiation was highly effective at 60 s.The MIC of mutant strain B1-413 was reduced from 125?l?mL~(-1)to 32?l? mL~(-1) compared with B1,the genetic attribute of which was stable through four generation cultivation.Conclusion The B1 was sensitive to microwave irradiation,and the higher anti-fungi active mutants can be easy obtained after the treatment of microwave irradiation.
