Protective effects of polypeptides from Chlamys farreri(PCF) on murine thymocytes damaged by ultraviolet ray
- VernacularTitle:扇贝多肽对紫外线辐照小鼠胸腺淋巴细胞的保护作用
- Author:
Chunling YAN
- Publication Type:Journal Article
- Keywords:
polypeptides from Chlamys farreri;
lymphocyte;
ultraviolet ray;
antioxidation.
- From:
Chinese Journal of Marine Drugs
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
To study the protective effects of PCF on thymocytes in the mice damaged by ultraviolet ray in vitro,The irradiation damage model of thymocytes was established.The indices of GSH-Px,SOD,MDA,T-AOC and the cell viability were determined by biochemical and MTT methods respectively.The mitochondria membrane potential was tested using flow cytometry(FCM).The results showed that PCF could enhance the activities of GSH-Px,SOD and increase the values of A-SAC and T-AOC in cells,while decrease the amounts of ROS,MDA. In addition,PCF could stabilize the mitochondria membrane potential.These results indicated that PCF had the protective effects on the thymocytes damage by UV irradiation,The mechanisms of the effects might be related to the elimination of oxygen free radical and increase of the activities of anti-oxidative enzymes in cells.
