Estimate a method of automatic extracting genomic DNA from large numbers of whole blood samples
- VernacularTitle:自动提取大样本全血基因组DNA方法的建立
- Author:
Changrong CHEN
;
Bin PEI
;
Jinzhen XIE
- Publication Type:Journal Article
- Keywords:
Genomic DNA;
Whole blood;
Automatic DNA extracting
- From:
Chinese Journal of Blood Transfusion
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To estimate an automatic DNA extracting method, and evaluate the quantity and quality of these DNA samples.Methods Genomic DNA was extracted automatically from blood samples by MiniPrep 75-Ⅱ workstation. The yield and purity of DNA samples were detected by UV-spectrophotometer, and the integrality of these DNA samples were measured by agarose gel electrophoresis.Results The yield of genomic DNA extracted from 100?l whole blood were (7.33?2.58)?g. The average purity of these DNA samples was 1.647?0.135 (A260/A280) and the molecular weight of them was about 21 kb.Conclusion High quality and intact genomic DNA can be extracted rapidly from whole blood by using MiniPrep 75-Ⅱ workstation, and these DNA samples were suitable for downstream molecular biology experiments.
