Analysis of Molecular Profiles Among Trypanozoon Species and Subspecies By MGE-PCR Method
- VernacularTitle:利用MGE-PCR技术研究锥虫亚属种间与亚种间的分子特性
- Author:
Fengjun LI
;
Jiayu ZHENG
;
Wanzhong JIA
;
Zhaorong LUN
- Publication Type:Journal Article
- Keywords:
Trypanozoon;
Trypanosoma evansi;
T. equiperdum;
T. b. brucei;
T. b. rhodesiense;
T. b. gambiense;
MGE-PCR;
Cluster analysis
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1997;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the relationship between genetic variability and evolution among Trypanosoma brucei (including T. b. brucei, T. b. rhodesiense and T. b. gambiense), T. evansi and T. equiperdum isolates. Methods Genomic DNAs of 26 trypanosome isolates were amplified by a mobile genetic elements (MGE)-PCR technique and cluster analysis was performed based on the molecular profiles with Neighbor-Joining method. Results The genetic variability among trypanosome isolates examined was obvious with an average genetic distance of 41.2% (ranged from 0 to 100%). Similarity coefficient among T. brucei isolates was 41.15% which was lower than that between T. evansi and T. equiperdum isolates. The closest relationship was found between T. evansi and T. brucei isolates with a similarity coefficient of 62.94%. The genetic variability between T. b. rhodesiense and T. b. brucei isolates was higher than that among T. b. gambiense isolates. Conclusion Species and subspecies in Trypanozoon displayed a higher genetic variability; T. equiperdum isolates collected from China and from South America, and T. evansi isolates from China and from South America, should have a similar origin.
