Protective effect of topiramate on neuronal apoptosis in hippocampus of rats with seizure
- VernacularTitle:托吡酯对癫癎大鼠海马神经元凋亡的保护作用
- Author:
Yuan XU
;
Shiyao BAO
;
Wen LI
- Publication Type:Journal Article
- Keywords:
epilepsy;
apoptosis;
topiramate
- From:
Journal of Clinical Neurology
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of topiramate(TPM) on neuronal apoptosis in rats with acute seizures.Methods We treated the PTZ-induced seizure rats with TPM at 80mg/(kg?d)(high-dose group) and 40mg/(kg?d)(middle-dose group) or physiological saline(control group) for 2 weeks.Neuronal apoptosis in CA_1 and CA_3 regions in hippocampus was identified by terminal deoxynucletidyl transferase-mediated dUTP-biotin in situ nick end labeling(TUNEL) assay.Results Two weeks following seizures,TUNEL-positive neurons were detected in CA_1 and CA_3 regions each group.The numbers of TUNEL-positive neurons in CA_1 and CA_3 of control group were(35.83?)4.58 and(36.83?)3.83,(23.50?)2.81 and(25.50?)3.72 of high-dose TPM group,(31.52?)3.43 and(32.35?)4.69 of middle-dose TPM group.There was a very significant difference between high-dose TPM group and control group(all(P)0.05).Conclusion High dose administration of TPM after experimental status epilepticus may attenuate seizure-induced hippocampal neuronal injury.
